This standard operating procedure (SOP) describes the process for evaluating the susceptibility of adult mosquito vectors to insecticides using the WHO bottle bioassay. The bottle bioassay should be used to evaluate vector susceptibility to insecticides that are unstable or cannot impregnate usual Whatman no. 1 filter papers. This SOP is for testing susceptibility to these insecticides and should not be used for insecticide growth regulators that impact mosquito fecundity/fertility. The procedure for testing insect growth regulators is presented in the SOP for evaluating the sterilizing properties of pyriproxyfen in adult female mosquitoes in WHO bottle bioassays. For insecticides that can impregnate filter papers, the SOP for WHO tube tests should be followed.

Download : https://apps.who.int/iris/rest/bitstreams/1412892/retrieve