This standard operating procedure (SOP) describes the process to follow for evaluating the susceptibility of adult mosquito vectors to insecticides using the WHO tube test. This bioassay is a direct response-to-exposure test, measuring mosquito mortality 24 hours after exposure to a known standard concentration of an insecticide (e.g. the discriminating concentration) for a period of 1 hour. This procedure should be followed for testing mosquito susceptibility to insecticides that can impregnate filter papers. For insecticides that are unstable on filter papers or that cannot be impregnated on filter papers, the WHO bottle bioassay procedure should be followed instead.

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